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Rabbit Nf κb P65 Polyclonal Antibody Hrp Peroxidase Conjugated Bioss Antibodies, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) We assessed T cell activation by measuring the expression of CD69 and CD25 on the cell surface. Purified CD4⁺ T cells from term and preterm neonates were either left unstimulated or stimulated under the indicated conditions for 72 hours. The percentages of CD69⁺ and CD25⁺ T cells were determined by flow cytometry. (B) For transcription factor activation, we evaluate phosphorylation of c-Jun and <t>p65</t> as markers of AP-1 and NF-κB activation, respectively. CBMCs were either left unstimulated or stimulated under the indicated conditions for 90 minutes (to measure c-Jun activation) or 60 minutes (for p65 activation). Mean fluorescence intensity (MFI) values for the indicated phosphoproteins were determined by flow cytometry. (C) MFI normalized values. The Wilcoxon test was used for paired samples, and the Mann–Whitney U test was used for unpaired samples. P values are shown above the error bars. * P < 0.05, ** P < 0.01. For the evaluation of CD69 and CD25 expression, the number of samples was as follows: vaginal delivery = 13, cesarean section = 9, and preterm birth = 3. For the evaluation of transcription factor activation, the sample sizes were: Natural birth = 7, Cesarean = 8, and Preterm = 5.
Anti Phospho P65, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) We assessed T cell activation by measuring the expression of CD69 and CD25 on the cell surface. Purified CD4⁺ T cells from term and preterm neonates were either left unstimulated or stimulated under the indicated conditions for 72 hours. The percentages of CD69⁺ and CD25⁺ T cells were determined by flow cytometry. (B) For transcription factor activation, we evaluate phosphorylation of c-Jun and <t>p65</t> as markers of AP-1 and NF-κB activation, respectively. CBMCs were either left unstimulated or stimulated under the indicated conditions for 90 minutes (to measure c-Jun activation) or 60 minutes (for p65 activation). Mean fluorescence intensity (MFI) values for the indicated phosphoproteins were determined by flow cytometry. (C) MFI normalized values. The Wilcoxon test was used for paired samples, and the Mann–Whitney U test was used for unpaired samples. P values are shown above the error bars. * P < 0.05, ** P < 0.01. For the evaluation of CD69 and CD25 expression, the number of samples was as follows: vaginal delivery = 13, cesarean section = 9, and preterm birth = 3. For the evaluation of transcription factor activation, the sample sizes were: Natural birth = 7, Cesarean = 8, and Preterm = 5.
P Nf κb P65, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) We assessed T cell activation by measuring the expression of CD69 and CD25 on the cell surface. Purified CD4⁺ T cells from term and preterm neonates were either left unstimulated or stimulated under the indicated conditions for 72 hours. The percentages of CD69⁺ and CD25⁺ T cells were determined by flow cytometry. (B) For transcription factor activation, we evaluate phosphorylation of c-Jun and <t>p65</t> as markers of AP-1 and NF-κB activation, respectively. CBMCs were either left unstimulated or stimulated under the indicated conditions for 90 minutes (to measure c-Jun activation) or 60 minutes (for p65 activation). Mean fluorescence intensity (MFI) values for the indicated phosphoproteins were determined by flow cytometry. (C) MFI normalized values. The Wilcoxon test was used for paired samples, and the Mann–Whitney U test was used for unpaired samples. P values are shown above the error bars. * P < 0.05, ** P < 0.01. For the evaluation of CD69 and CD25 expression, the number of samples was as follows: vaginal delivery = 13, cesarean section = 9, and preterm birth = 3. For the evaluation of transcription factor activation, the sample sizes were: Natural birth = 7, Cesarean = 8, and Preterm = 5.
Nf κb, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) We assessed T cell activation by measuring the expression of CD69 and CD25 on the cell surface. Purified CD4⁺ T cells from term and preterm neonates were either left unstimulated or stimulated under the indicated conditions for 72 hours. The percentages of CD69⁺ and CD25⁺ T cells were determined by flow cytometry. (B) For transcription factor activation, we evaluate phosphorylation of c-Jun and <t>p65</t> as markers of AP-1 and NF-κB activation, respectively. CBMCs were either left unstimulated or stimulated under the indicated conditions for 90 minutes (to measure c-Jun activation) or 60 minutes (for p65 activation). Mean fluorescence intensity (MFI) values for the indicated phosphoproteins were determined by flow cytometry. (C) MFI normalized values. The Wilcoxon test was used for paired samples, and the Mann–Whitney U test was used for unpaired samples. P values are shown above the error bars. * P < 0.05, ** P < 0.01. For the evaluation of CD69 and CD25 expression, the number of samples was as follows: vaginal delivery = 13, cesarean section = 9, and preterm birth = 3. For the evaluation of transcription factor activation, the sample sizes were: Natural birth = 7, Cesarean = 8, and Preterm = 5.
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(A) We assessed T cell activation by measuring the expression of CD69 and CD25 on the cell surface. Purified CD4⁺ T cells from term and preterm neonates were either left unstimulated or stimulated under the indicated conditions for 72 hours. The percentages of CD69⁺ and CD25⁺ T cells were determined by flow cytometry. (B) For transcription factor activation, we evaluate phosphorylation of c-Jun and <t>p65</t> as markers of AP-1 and NF-κB activation, respectively. CBMCs were either left unstimulated or stimulated under the indicated conditions for 90 minutes (to measure c-Jun activation) or 60 minutes (for p65 activation). Mean fluorescence intensity (MFI) values for the indicated phosphoproteins were determined by flow cytometry. (C) MFI normalized values. The Wilcoxon test was used for paired samples, and the Mann–Whitney U test was used for unpaired samples. P values are shown above the error bars. * P < 0.05, ** P < 0.01. For the evaluation of CD69 and CD25 expression, the number of samples was as follows: vaginal delivery = 13, cesarean section = 9, and preterm birth = 3. For the evaluation of transcription factor activation, the sample sizes were: Natural birth = 7, Cesarean = 8, and Preterm = 5.
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(A) We assessed T cell activation by measuring the expression of CD69 and CD25 on the cell surface. Purified CD4⁺ T cells from term and preterm neonates were either left unstimulated or stimulated under the indicated conditions for 72 hours. The percentages of CD69⁺ and CD25⁺ T cells were determined by flow cytometry. (B) For transcription factor activation, we evaluate phosphorylation of c-Jun and <t>p65</t> as markers of AP-1 and NF-κB activation, respectively. CBMCs were either left unstimulated or stimulated under the indicated conditions for 90 minutes (to measure c-Jun activation) or 60 minutes (for p65 activation). Mean fluorescence intensity (MFI) values for the indicated phosphoproteins were determined by flow cytometry. (C) MFI normalized values. The Wilcoxon test was used for paired samples, and the Mann–Whitney U test was used for unpaired samples. P values are shown above the error bars. * P < 0.05, ** P < 0.01. For the evaluation of CD69 and CD25 expression, the number of samples was as follows: vaginal delivery = 13, cesarean section = 9, and preterm birth = 3. For the evaluation of transcription factor activation, the sample sizes were: Natural birth = 7, Cesarean = 8, and Preterm = 5.
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mouse monoclonal anti nfκβ p65  (Sino Biological)
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Violin box plot showing the percentage of differentiated astrocytes (NDRG2+) that express the reactive astrocytes biomarkers pSTAT3 ( A ) and <t>NFκβ</t> <t>p65</t> ( E ) in cortical tissue from controls (n = 12) and DR-TLE patients (pSTAT3: n = 17; NFκβ p65: n = 16). B-D) Percentage of astrocytes co-expressing NDRG2 and pSTAT3 classified according to their DNA content. B ) % 2C astrocytes; C ) % 4C astrocytes; D ) % >4C astrocytes. F-H) Percentage of astrocytes co-expressing NDRG2 and NFκβ p65 classified according to their DNA content. F ) % 2C astrocytes; G ) % 4C astrocytes; H ) % >4C astrocytes. **p < 0.01, ***p < 0.001. Abbreviations: DR-TLE: drug-resistant temporal lobe epilepsy; NDRG2: N-Myc Downstream-Regulated Gene 2 Protein; p65: <t>p65</t> <t>subunit</t> of nuclear factor-κβ; pSTAT3: phosphorylated Signal Transducer and Activator of Transcription 3; 2C: diploid; 4C: tetraploid; >4C: Polyploid with a DNA content higher than 4C.
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(A) We assessed T cell activation by measuring the expression of CD69 and CD25 on the cell surface. Purified CD4⁺ T cells from term and preterm neonates were either left unstimulated or stimulated under the indicated conditions for 72 hours. The percentages of CD69⁺ and CD25⁺ T cells were determined by flow cytometry. (B) For transcription factor activation, we evaluate phosphorylation of c-Jun and p65 as markers of AP-1 and NF-κB activation, respectively. CBMCs were either left unstimulated or stimulated under the indicated conditions for 90 minutes (to measure c-Jun activation) or 60 minutes (for p65 activation). Mean fluorescence intensity (MFI) values for the indicated phosphoproteins were determined by flow cytometry. (C) MFI normalized values. The Wilcoxon test was used for paired samples, and the Mann–Whitney U test was used for unpaired samples. P values are shown above the error bars. * P < 0.05, ** P < 0.01. For the evaluation of CD69 and CD25 expression, the number of samples was as follows: vaginal delivery = 13, cesarean section = 9, and preterm birth = 3. For the evaluation of transcription factor activation, the sample sizes were: Natural birth = 7, Cesarean = 8, and Preterm = 5.

Journal: bioRxiv

Article Title: PREMATURE BIRTH AND CESAREAN SECTION AFFECT NEONATAL CD4 + T CELL GENE EXPRESSION AND CELLULAR FUNCTION

doi: 10.64898/2026.02.21.707199

Figure Lengend Snippet: (A) We assessed T cell activation by measuring the expression of CD69 and CD25 on the cell surface. Purified CD4⁺ T cells from term and preterm neonates were either left unstimulated or stimulated under the indicated conditions for 72 hours. The percentages of CD69⁺ and CD25⁺ T cells were determined by flow cytometry. (B) For transcription factor activation, we evaluate phosphorylation of c-Jun and p65 as markers of AP-1 and NF-κB activation, respectively. CBMCs were either left unstimulated or stimulated under the indicated conditions for 90 minutes (to measure c-Jun activation) or 60 minutes (for p65 activation). Mean fluorescence intensity (MFI) values for the indicated phosphoproteins were determined by flow cytometry. (C) MFI normalized values. The Wilcoxon test was used for paired samples, and the Mann–Whitney U test was used for unpaired samples. P values are shown above the error bars. * P < 0.05, ** P < 0.01. For the evaluation of CD69 and CD25 expression, the number of samples was as follows: vaginal delivery = 13, cesarean section = 9, and preterm birth = 3. For the evaluation of transcription factor activation, the sample sizes were: Natural birth = 7, Cesarean = 8, and Preterm = 5.

Article Snippet: We used anti–phospho c-Jun (Ser63, cat. GTX61170, GeneTex), secondary anti–rabbit FITC-conjugated antibody (GeneTex), and anti–phospho p65 (Ser529)–APC (Miltenyi Biotec).

Techniques: Activation Assay, Expressing, Purification, Flow Cytometry, Phospho-proteomics, Fluorescence, MANN-WHITNEY

Violin box plot showing the percentage of differentiated astrocytes (NDRG2+) that express the reactive astrocytes biomarkers pSTAT3 ( A ) and NFκβ p65 ( E ) in cortical tissue from controls (n = 12) and DR-TLE patients (pSTAT3: n = 17; NFκβ p65: n = 16). B-D) Percentage of astrocytes co-expressing NDRG2 and pSTAT3 classified according to their DNA content. B ) % 2C astrocytes; C ) % 4C astrocytes; D ) % >4C astrocytes. F-H) Percentage of astrocytes co-expressing NDRG2 and NFκβ p65 classified according to their DNA content. F ) % 2C astrocytes; G ) % 4C astrocytes; H ) % >4C astrocytes. **p < 0.01, ***p < 0.001. Abbreviations: DR-TLE: drug-resistant temporal lobe epilepsy; NDRG2: N-Myc Downstream-Regulated Gene 2 Protein; p65: p65 subunit of nuclear factor-κβ; pSTAT3: phosphorylated Signal Transducer and Activator of Transcription 3; 2C: diploid; 4C: tetraploid; >4C: Polyploid with a DNA content higher than 4C.

Journal: medRxiv

Article Title: Functional Profiling of Tetraploid Astrocytes in Drug-Resistant Temporal Lobe Epilepsy

doi: 10.64898/2026.01.30.26345206

Figure Lengend Snippet: Violin box plot showing the percentage of differentiated astrocytes (NDRG2+) that express the reactive astrocytes biomarkers pSTAT3 ( A ) and NFκβ p65 ( E ) in cortical tissue from controls (n = 12) and DR-TLE patients (pSTAT3: n = 17; NFκβ p65: n = 16). B-D) Percentage of astrocytes co-expressing NDRG2 and pSTAT3 classified according to their DNA content. B ) % 2C astrocytes; C ) % 4C astrocytes; D ) % >4C astrocytes. F-H) Percentage of astrocytes co-expressing NDRG2 and NFκβ p65 classified according to their DNA content. F ) % 2C astrocytes; G ) % 4C astrocytes; H ) % >4C astrocytes. **p < 0.01, ***p < 0.001. Abbreviations: DR-TLE: drug-resistant temporal lobe epilepsy; NDRG2: N-Myc Downstream-Regulated Gene 2 Protein; p65: p65 subunit of nuclear factor-κβ; pSTAT3: phosphorylated Signal Transducer and Activator of Transcription 3; 2C: diploid; 4C: tetraploid; >4C: Polyploid with a DNA content higher than 4C.

Article Snippet: Mouse monoclonal anti-NFκβ p65 (12054-MM04 Sino Biological, USA) and anti-pSTAT3 (sc-8059 Santa Cruz Biotechnology, USA) antibodies were both diluted 1:500.

Techniques: Expressing

Diagram of the functions played by tetraploid astrocytes. Tetraploid astrocytes express NF1A suggesting that they are involved in electric function. Tetraploid astrocytes present SOX9, a marker of glutamate transporter function and ALDH1L1, an enzyme involved in glucose metabolism. Some of the tetraploid astrocytes have markers of reactive astrocytes (NFκB p65 and pSTAT3). Abbreviation: GLT-1: glutamate transporter 1; NADPH: Nicotinamide adenine dinucleotide phosphate; NDRG2: N-Myc Downstream-Regulated Gene 2 Protein; NF1A: Nuclear factor 1; p65: p65 subunit of nuclear factor-κβ; SOX9: SRY-related high mobility group box gene 9; pSTAT3: phosphorylated Signal Transducer and Activator of Transcription 3; 4C: tetraploid.

Journal: medRxiv

Article Title: Functional Profiling of Tetraploid Astrocytes in Drug-Resistant Temporal Lobe Epilepsy

doi: 10.64898/2026.01.30.26345206

Figure Lengend Snippet: Diagram of the functions played by tetraploid astrocytes. Tetraploid astrocytes express NF1A suggesting that they are involved in electric function. Tetraploid astrocytes present SOX9, a marker of glutamate transporter function and ALDH1L1, an enzyme involved in glucose metabolism. Some of the tetraploid astrocytes have markers of reactive astrocytes (NFκB p65 and pSTAT3). Abbreviation: GLT-1: glutamate transporter 1; NADPH: Nicotinamide adenine dinucleotide phosphate; NDRG2: N-Myc Downstream-Regulated Gene 2 Protein; NF1A: Nuclear factor 1; p65: p65 subunit of nuclear factor-κβ; SOX9: SRY-related high mobility group box gene 9; pSTAT3: phosphorylated Signal Transducer and Activator of Transcription 3; 4C: tetraploid.

Article Snippet: Mouse monoclonal anti-NFκβ p65 (12054-MM04 Sino Biological, USA) and anti-pSTAT3 (sc-8059 Santa Cruz Biotechnology, USA) antibodies were both diluted 1:500.

Techniques: Marker